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flow cytometric analysis fresh human peripheral blood mononuclear cells pbmcs  (Beijing Solarbio Science)


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    Beijing Solarbio Science flow cytometric analysis fresh human peripheral blood mononuclear cells pbmcs
    Figure 2. Deficient NK cell function in IT patients. (A) Deficient NK cell cytotoxicity from IT patients. NK cell cytotoxicity was assessed in primary NK cells from IT, IA, and IN patients and healthy controls using a 51Cr-release assay with K562 target cells. (B) Results show the mean 6 SEM values in 19 patients and 5 controls. Values for IT patients were less than controls (P= 0.0384). (C) Positive correlation between NK cell cytotoxicity and the percentage of NK cells expressing NKG2D and 2B4. (D) Fresh <t>PBMCs</t> were stimulated with/without IL-12 as described in the Materials and Methods. After 16 h, IFN-c production was determined using flow cytometry by gating on CD32CD56+ NK cells. A representative dot plot displaying intracellular IFNc staining in NK cells with the subsets indicated is shown. (E) Cumulative data are shown. (F, G) Fresh PBMCs from patients before treatment (black) or after treatment (white) were stimulated with IL-12 for 16 h. IFNc (F) and CD107a (G) production was determined using flow cytometry by gating on CD32CD56+ NK cells. (H) Induction of ex vivo NK cell cytotoxic activity after in vivo administration of antiviral treatment with nucleoside analogues until ALT reached normal levels. NK cytotoxicity was determined ex vivo by measuring the lysis of 51Cr-labelled target cells before treatment (red) or after treatment (green). Cumulative data are shown. doi:10.1371/journal.ppat.1002594.g002
    Flow Cytometric Analysis Fresh Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometric+analysis+fresh+human+peripheral+blood+mononuclear+cells+pbmcs/Human+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution/pm22438812-313-0-17
    Average 96 stars, based on 295 article reviews
    flow cytometric analysis fresh human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2026-11
    96/100 stars

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    1) Product Images from "TGF-β1 down-regulation of NKG2D/DAP10 and 2B4/SAP expression on human NK cells contributes to HBV persistence."

    Article Title: TGF-β1 down-regulation of NKG2D/DAP10 and 2B4/SAP expression on human NK cells contributes to HBV persistence.

    Journal: PLoS pathogens

    doi: 10.1371/journal.ppat.1002594

    Figure 2. Deficient NK cell function in IT patients. (A) Deficient NK cell cytotoxicity from IT patients. NK cell cytotoxicity was assessed in primary NK cells from IT, IA, and IN patients and healthy controls using a 51Cr-release assay with K562 target cells. (B) Results show the mean 6 SEM values in 19 patients and 5 controls. Values for IT patients were less than controls (P= 0.0384). (C) Positive correlation between NK cell cytotoxicity and the percentage of NK cells expressing NKG2D and 2B4. (D) Fresh PBMCs were stimulated with/without IL-12 as described in the Materials and Methods. After 16 h, IFN-c production was determined using flow cytometry by gating on CD32CD56+ NK cells. A representative dot plot displaying intracellular IFNc staining in NK cells with the subsets indicated is shown. (E) Cumulative data are shown. (F, G) Fresh PBMCs from patients before treatment (black) or after treatment (white) were stimulated with IL-12 for 16 h. IFNc (F) and CD107a (G) production was determined using flow cytometry by gating on CD32CD56+ NK cells. (H) Induction of ex vivo NK cell cytotoxic activity after in vivo administration of antiviral treatment with nucleoside analogues until ALT reached normal levels. NK cytotoxicity was determined ex vivo by measuring the lysis of 51Cr-labelled target cells before treatment (red) or after treatment (green). Cumulative data are shown. doi:10.1371/journal.ppat.1002594.g002
    Figure Legend Snippet: Figure 2. Deficient NK cell function in IT patients. (A) Deficient NK cell cytotoxicity from IT patients. NK cell cytotoxicity was assessed in primary NK cells from IT, IA, and IN patients and healthy controls using a 51Cr-release assay with K562 target cells. (B) Results show the mean 6 SEM values in 19 patients and 5 controls. Values for IT patients were less than controls (P= 0.0384). (C) Positive correlation between NK cell cytotoxicity and the percentage of NK cells expressing NKG2D and 2B4. (D) Fresh PBMCs were stimulated with/without IL-12 as described in the Materials and Methods. After 16 h, IFN-c production was determined using flow cytometry by gating on CD32CD56+ NK cells. A representative dot plot displaying intracellular IFNc staining in NK cells with the subsets indicated is shown. (E) Cumulative data are shown. (F, G) Fresh PBMCs from patients before treatment (black) or after treatment (white) were stimulated with IL-12 for 16 h. IFNc (F) and CD107a (G) production was determined using flow cytometry by gating on CD32CD56+ NK cells. (H) Induction of ex vivo NK cell cytotoxic activity after in vivo administration of antiviral treatment with nucleoside analogues until ALT reached normal levels. NK cytotoxicity was determined ex vivo by measuring the lysis of 51Cr-labelled target cells before treatment (red) or after treatment (green). Cumulative data are shown. doi:10.1371/journal.ppat.1002594.g002

    Techniques Used: Cell Function Assay, Release Assay, Expressing, Flow Cytometry, Staining, Ex Vivo, Activity Assay, In Vivo, Analogues, Lysis

    Related Articles

    Isolation:

    Article Title: TGF-β1 down-regulation of NKG2D/DAP10 and 2B4/SAP expression on human NK cells contributes to HBV persistence.
    Article Snippet: .. Flow cytometric analysis Fresh human peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood by Ficoll-Isopaque (Solarbio, China) gradient centrifugation. .. NK cells were freshly purified from PBMCs by negative selection using magnetic microbead separation kits (Miltenyi Biotec), resulting in .90% purity.

    Gradient Centrifugation:

    Article Title: TGF-β1 down-regulation of NKG2D/DAP10 and 2B4/SAP expression on human NK cells contributes to HBV persistence.
    Article Snippet: .. Flow cytometric analysis Fresh human peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood by Ficoll-Isopaque (Solarbio, China) gradient centrifugation. .. NK cells were freshly purified from PBMCs by negative selection using magnetic microbead separation kits (Miltenyi Biotec), resulting in .90% purity.



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    flow cytometric analysis fresh human peripheral blood mononuclear cells pbmcs  (Beijing Solarbio Science)
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    Beijing Solarbio Science flow cytometric analysis fresh human peripheral blood mononuclear cells pbmcs
    Figure 2. Deficient NK cell function in IT patients. (A) Deficient NK cell cytotoxicity from IT patients. NK cell cytotoxicity was assessed in primary NK cells from IT, IA, and IN patients and healthy controls using a 51Cr-release assay with K562 target cells. (B) Results show the mean 6 SEM values in 19 patients and 5 controls. Values for IT patients were less than controls (P= 0.0384). (C) Positive correlation between NK cell cytotoxicity and the percentage of NK cells expressing NKG2D and 2B4. (D) Fresh <t>PBMCs</t> were stimulated with/without IL-12 as described in the Materials and Methods. After 16 h, IFN-c production was determined using flow cytometry by gating on CD32CD56+ NK cells. A representative dot plot displaying intracellular IFNc staining in NK cells with the subsets indicated is shown. (E) Cumulative data are shown. (F, G) Fresh PBMCs from patients before treatment (black) or after treatment (white) were stimulated with IL-12 for 16 h. IFNc (F) and CD107a (G) production was determined using flow cytometry by gating on CD32CD56+ NK cells. (H) Induction of ex vivo NK cell cytotoxic activity after in vivo administration of antiviral treatment with nucleoside analogues until ALT reached normal levels. NK cytotoxicity was determined ex vivo by measuring the lysis of 51Cr-labelled target cells before treatment (red) or after treatment (green). Cumulative data are shown. doi:10.1371/journal.ppat.1002594.g002
    Flow Cytometric Analysis Fresh Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometric+analysis+fresh+human+peripheral+blood+mononuclear+cells+pbmcs/Human+Peripheral+Blood+Mononuclear+Cell+Isolation+Solution/pm22438812-313-0-17
    Average 96 stars, based on 1 article reviews
    flow cytometric analysis fresh human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier

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    Figure 2. Deficient NK cell function in IT patients. (A) Deficient NK cell cytotoxicity from IT patients. NK cell cytotoxicity was assessed in primary NK cells from IT, IA, and IN patients and healthy controls using a 51Cr-release assay with K562 target cells. (B) Results show the mean 6 SEM values in 19 patients and 5 controls. Values for IT patients were less than controls (P= 0.0384). (C) Positive correlation between NK cell cytotoxicity and the percentage of NK cells expressing NKG2D and 2B4. (D) Fresh PBMCs were stimulated with/without IL-12 as described in the Materials and Methods. After 16 h, IFN-c production was determined using flow cytometry by gating on CD32CD56+ NK cells. A representative dot plot displaying intracellular IFNc staining in NK cells with the subsets indicated is shown. (E) Cumulative data are shown. (F, G) Fresh PBMCs from patients before treatment (black) or after treatment (white) were stimulated with IL-12 for 16 h. IFNc (F) and CD107a (G) production was determined using flow cytometry by gating on CD32CD56+ NK cells. (H) Induction of ex vivo NK cell cytotoxic activity after in vivo administration of antiviral treatment with nucleoside analogues until ALT reached normal levels. NK cytotoxicity was determined ex vivo by measuring the lysis of 51Cr-labelled target cells before treatment (red) or after treatment (green). Cumulative data are shown. doi:10.1371/journal.ppat.1002594.g002

    Journal: PLoS pathogens

    Article Title: TGF-β1 down-regulation of NKG2D/DAP10 and 2B4/SAP expression on human NK cells contributes to HBV persistence.

    doi: 10.1371/journal.ppat.1002594

    Figure Lengend Snippet: Figure 2. Deficient NK cell function in IT patients. (A) Deficient NK cell cytotoxicity from IT patients. NK cell cytotoxicity was assessed in primary NK cells from IT, IA, and IN patients and healthy controls using a 51Cr-release assay with K562 target cells. (B) Results show the mean 6 SEM values in 19 patients and 5 controls. Values for IT patients were less than controls (P= 0.0384). (C) Positive correlation between NK cell cytotoxicity and the percentage of NK cells expressing NKG2D and 2B4. (D) Fresh PBMCs were stimulated with/without IL-12 as described in the Materials and Methods. After 16 h, IFN-c production was determined using flow cytometry by gating on CD32CD56+ NK cells. A representative dot plot displaying intracellular IFNc staining in NK cells with the subsets indicated is shown. (E) Cumulative data are shown. (F, G) Fresh PBMCs from patients before treatment (black) or after treatment (white) were stimulated with IL-12 for 16 h. IFNc (F) and CD107a (G) production was determined using flow cytometry by gating on CD32CD56+ NK cells. (H) Induction of ex vivo NK cell cytotoxic activity after in vivo administration of antiviral treatment with nucleoside analogues until ALT reached normal levels. NK cytotoxicity was determined ex vivo by measuring the lysis of 51Cr-labelled target cells before treatment (red) or after treatment (green). Cumulative data are shown. doi:10.1371/journal.ppat.1002594.g002

    Article Snippet: Flow cytometric analysis Fresh human peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood by Ficoll-Isopaque (Solarbio, China) gradient centrifugation.

    Techniques: Cell Function Assay, Release Assay, Expressing, Flow Cytometry, Staining, Ex Vivo, Activity Assay, In Vivo, Analogues, Lysis